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KMID : 0377519810060010113
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Chung-Ang Journal of Medicine
1981 Volume.6 No. 1 p.113 ~ p.120
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Measurement Conditions for Microanalysis of Inorganic Mercury to Improve Sensitivety and Precision
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Choi Ho-Chun
Chung Kyou-Chull
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Abstract
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This study was designed to define an optimal conditions for microanalysis of inorganic mercury by dithizone calorimetric methods now in use in order to improve its sensitivity and precision of the analysis. The optimal analytical conditions so far disclosed in this experiment were as follows: The mercury-dithizonate complex was extracted with chloroform as an organic mercury dithizonate, which exhibited an absorption maximum at 490 nm. The acidic aquous solution was to be prepared to contain 0.25 N sulfuric acid, Macllvain’s buffer and 40% (w/v) potassium bromide solution with ratio of 5:1:1, pH of the acidic solution was to be adjusted to 6, and the total volume of the solution was considered to be optimal in the range of 70-120ml. Although heavy metals other than mercury also formed the stable complexes with dithizone and interfered with the detection of mercury, it was demonstrated by using copper ion that the interfering effect was best controlled by 40-50% (w/v) potassium bromide solution. Application of the above mentioned standard method reduced the likelihood of expeimental errors and improved the accuracy and sensitivity of the analytical method. It was concluded that the detection range was 3-20㎍Hg, sensitivity 95-105% and precision was as low as 5% in terms of coefficient of variation.
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